Microarray | biology | Dallas County Community College District

1. Genomics is the scrutinize of:

a. The construction and employment   of mutations and how they diversify genetic traits.

b. Genes and the DNA followings   betwixt genes and how they indicate harvest.

c. The notice granted by   computer programs which analyzes mRNA.

d. The ethnical genome as paralleld   to other vertebrate genomes.


2. Microarrays are a very suited dupe in genomics owing they:

a. Acceleration scientists scrutinize   intergenetic DNA by separating it from genes.

b. Provide a singular defender   region for polymerase chain reactions.

c. Grant scientists to scrutinize   thousands of genes all at uniformly.

d. Decrease the age it takes   for scientists to constitute copies of DNA.


3. Generally,   every cell in our organization contains the similar 20,000 (or so) genes. However, cells in our organization are opposed   from each other owing they:

a. Keep   opposed genes turned “on” or “off” to remain opposed employments.

b. Contain   opposed copies of genes for opposed employments.

c. Provide   opposed nucleotide disesteemeds for each harvestal employment.

d. Employment   differently fixed on varying proteomics.


4. How can   scientists indicate the employment of or disagreements betwixt cell patterns? They can scrutinize the:

a. Number of   nucleotide disesteemeds in genes versus intergenetic followings.

b. Amount of   mRNA explicit for each gene in a cell pattern, and then parallel that   notice betwixt cell patterns.

c. Amount of   mutations betwixt genes in the intergenetic spaces.

d. Number of   tRNA copies for a debottom cell pattern.


5. How is a   microarray constrained? In each want,   there are:

a. Copies of   all the genes for an organism.

b. Multiple copies   of one gene; each want has copies for a opposed gene.

c. Multiple   copies of intergenetic followings, which oblige to genes in the scantlings.

d. Copies of   intergenetic followings, which prefer the replication of DNA in a scantling.


6. The   experiment that begins in Chapter 3 of the mannerism seeks to response the   question:

a. What is   the disagreement betwixt intergenetic spaces in cancer cells versus sound   cells?

b. Why do   opposed cell patterns pointed opposed amounts of mRNA?

c. How do   opposed cancer cells product opposed mutations?

d. What is   the disagreement betwixt sound cells and cancer cells?


7. Why can’t   doctors use cell apparition to diagnose cancer?

a. Not all   cancer cells behold opposed from sound cells.

b. Cancer   cells are too fine to scrutinize using cell apparition.

c. Not all   cancer cells are powerful to be biopsied from the organization.

d. Cancer   cells shift apparition when enslaved out of the organization.


8. In the   experiment, a solvent is acquired to each cell pattern (sound cells and cancer   cells). After the scantling tube   containing each cell pattern is qualified on the commotion, the RNA is disconnected from   the cessation of the scantling in a centrifuge. Why does DNA arrange to the profound of the tube and RNA doesn’t?

a. RNA is   much longer than DNA.

b. RNA is   attached to proteins that acceleration it remain in discerption.

c. DNA is   attached to biomolecules that scrutinize it down and acceleration it arrange to the profound.

d. DNA is   much longer than RNA.


9. What   feature does mRNA keep that tRNA and rRNA do not? mRNA always:

a. Contains a   GABA box.

b. Contains a   TATA following.

c. Ends after a while   a G bottom.

d. Ends after a while   a poly-A bottom.


10. How do   the beads in the shaft severed mRNA from all other RNA? The beads contain:

a. Sequences   that magnetically severed the mRNA.

b. A   glue-like body conservative from spider webs.

c. mRNA   contains a Poly A bottom that obliges to Poly-T’s.

d. It is   randomly disconnected.


11. After you   isolate mRNA, you keep to constitute a DNA portraiture. Why can’t we upright use mRNA?

a. DNA is   much more stpowerful than mRNA.

b. We keep to   add a fluorescent label that accomplish grant us to see the scantling.

c. mRNA accomplish   eventually transmute into tRNA making it impracticable.

d. A and B


12.   Scientists circumvent hybridization the key to microarrays. Hybridization occurs when:

a. Two   complimentary strands of DNA from opposed sources oblige to each other.

b. Poly-A   tails oblige to Poly-Ts.

c. Opposed   species interbreed and constitute new DNA disesteemed pairings.

d. Two   strands of selfsame DNA oblige after a whileout using the unwritten nucleotide pairs.


13. When you   scan the microarray in the scanner, the facts pretext some black wants. What do these enact?

a. The DNA   that has been replicated in sound cells.

b. The mRNA   that was washed loose in the washing discerption.

c. The DNA   that was not transcribed and explicit in sound cells.

d. The mRNA   that was not jump by Oligo-d-tails in the beads.


14. When you   scan the microarray in the scanner, some wants are yellow and enact   places where the gene was explicit in twain sound and cancer cells. These wants judge us:

a. Where to   behold for mutations.

b. Where DNA   hybridized in cancer cells.

c. That DNA   expression didn’t shift in these genes when cancer occurred.

d. That the   microarray didn’t effect in these genes.


15. In our   example, gene 6219 mRNA is made in twain sound and cancerous cells; notwithstanding,   proteins are solely translated from that mRNA in sound cells. Microarray anatomy:

a. Shows us   this want by making yellow wants.

b. Cannot   pretext us this want, which is a secretiveness of this pattern of anatomy.

c. Pretext us   this want by making red wants.

d. Cannot   pretext us this want, which is a profit of this pattern of anatomy.

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